CENTER FOR TUBERCULOSIS RESEARCH

PROFILE

The Center for Tuberculosis Research (CTBR) is a unique collaborative Research Center in the Department of Microbiology of the Nigerian Institute of Medical Research. The first National Tuberculosis Reference Laboratory in Nigeria is domiciled within this Center.

The Center for Tuberculosis Research (CTBR), is a unique collaborative Research Center founded in July 2017 in the Department of Microbiology of the Nigerian Institute of Medical Research.  The first National Tuberculosis Reference Laboratory in Nigeria is domiciled within this Center. This Center contributes to the WHO End TB Strategy programme through research, service and training. CTBR conducts a range of multi-disciplinary research and training by dedicated scientists and support staff in collaboration with universities, public and private sector organizations within the sub-region and internationally.

Research

Our research is focused on tuberculosis (TB) epidemiology, drug resistance, evaluation of novel diagnostics, genomics and laboratory management of MDRTB patients. Current research is supported by the Nigerian Government (Nigerian Institute of Medical Research and International organization (West African Node of Excellence for TB, AIDS and Malaria [WANETAM]. We also access grants for our research activities.

 Research Team:

CTBR is comprised of four Research Scientists, five Laboratory Scientists, two Laboratory Technologists, two Scientific Officers and four adhoc scientists and a few support staff.

S/NNAMEDESIGNATION
RESEARCH FELLOW
1Mrs. C. N. Kunle-OpeResearch Fellow II/Head
2Dr. N. N. OnyejepuChief Research Fellow
3Dr. (Mrs.) O. F. Davies-BolorunduroResearch Fellow I
4Mrs. I. T. NdubuisiResearch Fellow II
MEDICAL LABORATORY SCIENTIST
5Mrs. N. E. TochukwuChief Medical Laboratory Scientist
6Mr. A. A. AdesesanChief Medical Laboratory Scientist
7Rev. Sr. C. O. EjezieChief Medical Laboratory Scientist
8Mrs. M. A. A. AdetunjiAsst. Chief Med. Laboratory Scientist
9Mr. S. J. NduagaAsst. Chief Med. Laboratory Scientist
SCIENCE LABORATORY SCIENTIST
10Mrs. I. O. Edu-muyideenChief Science Laboratory Technologist
11Mrs. A. T. AbiodunPrincipal Laboratory Technologist
SCIENTIFIC OFFICER
12Mrs. B. O. AcholoScientific Officer II
13Mr. G. R. LawalScientific Officer II
ADMINISTRATIVE STAFF
14Mr. D. AirekhomeAdministrative Officer
15Mrs. C. J. AlaribeClerical Officer I
16Mrs. L. AllenClerical Officer I
ADHOC STAFF
17Mr. K. AtoeSenior Programme Officer (IHVN)
18Mrs. C. C. OkorieMedical Laboratory Scientist (IHVN)
19Miss B. N. OmolieMedical Laboratory Scientist (IHVN)
PROJECT STAFF
20Miss O. K. MustaphaScience Laboratory Technologist
21Miss T. S. AllisonData Officer
ADJUNCT PROFESSOR
22Prof. S. I. B. Cadmus

ACHIEVEMENT

  • Evaluated national treatment options for DRTB
  • Successfully re-assessed by SANAS for ISO 15189 accreditation
  • Good performance in External Quality Assurance programmes
  • Received equipment support from IHVN

The Centre has research groups to improve research output namely:

 TB Diagnostic & Epidemiology Research Group – conducts research into:

  • TB Diagnosis
    • Characterizing, tracking trends and profile of Mycobacterium tuberculosis Complex (MTBC) including Mycobacterium bovis and Non-tuberculous mycobacteria (NTM).
  • TB Epidemiology
    • Molecular epidemiology of TB
    • Genetic diversity of TB
  • Research on diagnostic and monitoring tools
    • Method verification and comparison of diagnostic laboratory methods
    • Evaluation of novel diagnostics

(For more details contact Mrs. Kunle-Ope C. Nchiomakunleope@gmail.com, cnkunle-ope@nimr.gov.ng)

 TB Drug Resistance and Pharmocogenomics Research Group – conducts research into:

  • Drug resistance of mycobacteria
  • Antimycobacterial natural products
  • Pharmocogenomics

(For more details contact Dr. Davis-Bolourunduro O. F.   bizdave1@gmail.com, davies-bolorunduro@nimr.gov.ng)

 Ongoing Research Activities

  • Triage Test for All Oral DR-TB Regimen (TRIAD) (In Collaboration with Institute of Human Virology, Abuja, Nigeria)
  • Desk Review with Whole Genome Sequencing of Mycobacterium Tuberculosis
  • Correlation of Clinical Outcomes of Patients on Anti-Tuberculosis Regimen
  • Exploration of Marine Bacterial Community Genomics in Nigeria Coastal Waters for Prospective Novel Drugs and Bio-pharmaceutics (In Collaboration with Microbiology Department, University of Lagos)
  • Phase 2 Sequencing of Drug Resistant TB (REST HUB) Project – CTBR (In collaboration with In Collaboration with US CDC and Institute of Human Virology, Abuja, Nigeria)
  • Genomic Information Surveillance on Drug-resistant Mycobacterium tuberculosis Isolates from Nigeria/TB-Portal – CTBR (In Collaboration with Adjunct Professor)
  • Transposing multidrug-resistant M. tuberculosis molecular and genomic epidemiology to Public Health and Product Development in Nigeria (In Collaboration with University of Lisbon, Portugal)

 Completed Research Activities

  • Phase 1 Sequencing of Drug Resistant TB (REST HUB) Project – (In collaboration with US CDC and Institute of Human Virology, Abuja, Nigeria)
  • Whole Genome Sequencing for Investigating Mycobacterium Tuberculosis Transmission, Genetic Diversity and Multi-Drug Resistance Prediction in Lagos, Nigeria – (In Collaboration with Redeemer University)
  • Molecular Epidemiology and Multidrug Resistance Mycobacterium tuberculosis Complex
  • Susceptibility Testing of Delamanid and Bedaquinine using BACTEC MGIT 960 – (In collaboration with Institute of Human Virology, Abuja, Nigeria and Nation TB Control Programme)
  • Diagnosis of TB and Management of Paediatric TB (EDCTP-WANETAM Grant)
  • Study on the Evaluation of Respiratory Tract Bacterial Co-infections with COVID-19 Severity in Lagos, Nigeria
  • Bioprospecting for Antituberculous Potentials of Culturable Marine Actinomycetes from Lagos Lagoon Sediments

 Grant Awards

  • EDCTP-WANETAM-3 Grant – Dr. Onyejepu N.
  • HORIZON-JU-GH-EDCTP3-2022-CALL 1-01-03 – Dr. Onyejepu N.
  • NIMR Innovation Grant – Dr. Onyejepu N.
  • Postdoc in Mahidol University, Thailand – Dr. Davies-Bolorunduro O.F
  • EDCTP-WANETAM-2 Grant – Dr. Onyejepu N.
  • EDCTP TMA Award – Dr. Onyejepu N.

Laboratory services: National Tuberculosis Reference Laboratory

The Center offers diagnostic services for pulmonary and extra-pulmonary TB using both conventional and molecular methodologies under quality management system, good clinical and laboratory practice (GCLP). The CTBR is equipped with biosafety level 2 and 3 laboratories and a molecular diagnostic suite. We also prepare quality panels for public and private laboratories on TB microscopy and geneXpert. Our service is supported by the Nigerian Government (National Tuberculosis and Leprosy Control Programme), implementing partners (Institute of Human Virology, Nigeria) and Tuberculosis Supra Reference Laboratory, Milan, Italy, Tuberculosis Supra Reference Laboratory, Antwerp, Belgium and Tuberculosis Supra Reference Laboratory, Cotonou, Benin).

In November 2019, CTBR obtained international accreditation to ISO15189 by South Africa National System (SANAS) on TB Microscopy and PCR-TB Identification and Drug Susceptibility Tests (GeneXpert and Hain). In January 2022, the Centre was successfully re-assed and the scope of accreditation was expanded to include TB Culture and Drug Susceptibility Testing.

PRICE LIST FOR TB TEST

S/NTESTPRICE (₦)
DIAGNOSIS USING CONVENTIONAL METHODS
1Sputum Smear Microscopy using Ziehl Nelsen (ZN) Stain5,000.00
2Sputum Smear Microscopy using Fluorescence (FM) Stain5,000.00
3Sputum Smear Microscopy and Culture on Solid Media (Lowenstein Jensen)10,000.00
4Sputum Smear Microscopy and Culture on Liquid Media (MGIT BACTEC 960 TB System) Only30,000.00
5Sputum Smear Microscopy, Culture and Drug Susceptibility Testing (DST) on Solid Media (Lowenstein Jensen) for First Line Drugs using Proportion Method25,000.00
6Sputum Smear Microscopy, Culture and Drug Susceptibility Testing (DST) on Solid Media (Lowenstein Jensen) for First- and Second-Line Drugs using Proportion Method30,000.00
7Sputum Smear Microscopy, Culture and Drug Susceptibility Testing (DST) in Liquid Media (MGIT BACTEC 960 TB System) for First Line Drugs Only40,000.00
8Sputum Smear Microscopy, Culture and Drug Susceptibility Testing (DST) in liquid media (MGIT BACTEC 960 TB System) for First- and Second-Line Drugs50,000.00
DIAGNOSIS USING MOLECULAR METHODS
9Diagnosis of TB and Detection of resistance to Rifampicin from Sputum/Stool Samples using GeneXpert10,000.00
10Diagnosis of TB and Detection of resistance to First Line Anti-TB Drugs (Rifampicin and Isoniazid) using Hain GenoType MTBDRplus (Line Probe Assay) Only30,000.00
11Diagnosis of TB and Detection of resistance First Line (Rifampicin and Isoniazid) and Second Line (Fluoroquinolones and Aminoglycosides) Anti-TB Drugs using Hain GenoType MTBDRplus (Line Probe Assay)60,000.00
12Species identification of Mycobacterium tuberculosis and other mycobacteria other than Mycobacterium tuberculosis using Hain GenoType CM/AS (Line Probe Assay)30,000.00
13Quality Panel Preparation for AFB Microscopy & GeneXpertSubject to negotiation
 
Data Management Services

Data management services are provided to clients and the cost depends on the number of data units and duration needed.

NB: Cost of a data services will be based on the sample size multiplied by the unit price with 10% concession for data more than 1 year.

S/NDATAUNIT PRICE BULK PRICE
1.Data on GeneXpertN5,000Sample Size X Unit Price
2.Data on AFB MicroscopyN 5000Sample Size X Unit Price
3.

Data on

First-line LPA

Second line LPA

Both first and second-line LPA

 

N8000

N8000

N15000

 

Sample Size x Unit Price

Sample Size x Unit Price

Sample Size X Unit Price.

4.

Data on Culture & DST

First-line drugs

Second-line drugs

Both first and second-line drugs

 

N8000

N8000

N15000

 

Sample Size x Unit Price

Sample Size x Unit Price

Sample Size x Unit Price

 

Biorespository

The Center has characterized mycobacterial collections in her bio-repository which can be accessed for research. Terms and condition apply.

 Training Programme:

The Centre utilizes its facilities for training of research scientists, and other organizations involved in Mycobacterium tuberculosis complex (MTBC) and other mycobacteria projects. We also provide hands on training for students on Internship and Industrial Training on TB Diagnostics as listed below:

  • AFB Microscopy using light and fluorescence microscope
  • GeneXpert
  • Line Probe Assay (LPA) – First- & Second-Line Anti-TB Drugs
  • Culture using solid (Loweinstein Jenseen media) and liquid (BACTEC MGIT 960 System)
  • Indentification of TB isolates using conventional biochemical tests and molecular methods
  • Drug Susceptibility Testing (DST) using solid (Loweinstein Jenseen media) and liquid (BACTEC MGIT 960 System) – First- & Second-Line Anti-TB Drugs
  • MIRU-VNTR Typing
  • Sequencing

We also provide training on the following:

  • Biosafety training
  • Advanced tuberculosis reasearch for health professional and post graduate students
  • Establising and maintaining quality assured TB laboratory services for public and private health practitioners
  • Diagnosis, treatment and conduct of research on multi drug resistant TB (MDR-TB)
 Other Services provided by the Center:
  • Quality audit for laboratories using SLPTA checklist
  • Monitoring and supervision for public and private laboratories
  • Technical assistance for public and private laboratories
  • Mentorship on Quality Management Systems for laboratories preparing for accreditation

Contact us:

E-mail: ctbr.micro@nimr.gov.ng

Kunle-Ope Chioma Nneka

Head/Laboratory Manager, CTBR

Miss Opeyemi Mustapha

STAFF OF THE MONTH – JUNE, 2023
(For meeting up with Test Turn-Around Time (TAT) on Genexpert bench)

Biosafety Level 3 Laboratory Suite

Sample Processing Room in Biosafety Level 3 Laboratory Suite

Sample Processing Room in Biosafety Level 2 Laboratory Suite

GeneXpert Room in Biosafety Level 2 Laboratory Suite

Molecular Suite for Molecular Diagnosis of TB

GeneXpert Room in Biosafety Level 2 Laboratory Suite